Đặng Văn Đông, Đỗ Minh Phú, Đoàn Thị Thuỳ Vân, Trịnh Khắc Quang (2009), Nghiên cứu hoàn thiện quy trình công nghệ nhân giống hoa lily bằng phương pháp nuôi cấy in vitro.

Đặng Văn Đông, Đỗ Minh Phú, Đoàn Thị Thuỳ Vân, Trịnh Khắc Quang (2009), Nghiên cứu hoàn thiện quy trình công nghệ nhân giống hoa lily bằng phương pháp nuôi cấy in vitro. Tạp chí Khoa học và Công nghệ Nông nghiệp Việt Nam số 3 tháng (12)/2009, tr 21-26.

SUMMARY

Improving lily sorbone propagation technology by in vitro culture method The present invention belongs to the field of biotechnology, and is tissue culture method to propagating great amount of Sorbone lily seedlings. Sorbone lily scale as explant, after sterilization and cutting into blocks, is inoculated on culture medium with different plant hormones to induce callus and cespitose bud; callus and cespitose bud are proliferated in secondary culture medium until reaching seedling height of 5 cm and forming base bulb; and the seedling is transplanted in grown bulb medium. The created and proliferation budlb culture medium includes MS supplemented NAA 0.01 mg/L and BAP 0.4 mg/L. The grown culture medium includes MS NAA 0.2 mg/L and BAP 0.03 mg/L. The culture conditions include temperature of 25 - 26 deg.C, illuminance of 2000 - 2200 lux, lighting 12 hr/day and pH 5.8.

Keywords: Biotechnology, tissue culture, callus, bulb, hormones.